The outgoing daytime queen greets 20,000 fans at Chicago's United Center.
"Today you are surrounded by nothing but love," the Oscar winner told Oprah.
The actor walked onstage to the Mission: Impossible theme.
The soul singer joined Josh Groban for "Over the Rainbow."
The Material Girl observed that Oprah is "still kicking ass."
The teen was 9 the first time she appeared on The Oprah Winfrey Show.
The admiring trio thanked Oprah for the strides she's taken for women everywhere.
The diva rocked the house (twice, thanks to technical difficulties) with "Run the World (Girls)."
The ABC News anchor announced that 25,000 oak trees were being planted in Oprah's honor.
Despite being in the middle of her own drama, the former first lady of California is a big enough Oprah fan to share in the lovefest.
The box-office star and wife Jada Pinkett Smith played hosts for the second half of the taping.
The comedian obviously felt it was important to dress up for the occasion. Unlike Beyoncé, he wore pants.
The poet read a new work written specially for the occasion, while the Grammy winner accompanied her on piano.
A healthy-looking Queen of Soul pays musical tribute to the Queen of Daytime.
Next year's Oscars can only hope for this sort of star power.
MORE PHOTOS: Oprah's Memorable Moments