Several singers tweeted in support of Underwood's heartfelt tribute, including Chris Young and Linda Davis.
Tonight marks the former American Idol star's 10th time co-hosting the Country Music Association Awards alongside the one and only Brad Paisley.
Earlier in the night, the songstress showed off her singing chops and her signature over-the-top outfit changes as she presented several awards and got the night off to a jam-packed start.
But while the evening is filled with fun and fancy frocks, things took a serious note during the monologue. In fact, Paisley told the crowd that tonight was about more than just shiny awards and pretty gowns.
"The way we see it, the best way to honor our fans is to play our music loud and proud. Our music lifts people up."
He added, "This year's show is dedicated to all those we've lost and all those still healing."