Glee Does "Don't Stop Believin'" With Lea Michele, Chris Colfer, Matthew Morrison and More—Hear It Now!

It's Glee's signature song!

Since 2009's one-of-a-kind pilot, the entire cast of the musical hit series have sung Journey's "Don't Stop Believin'" a grand total of three times in five seasons. (A quick refresher: The pilot, the first Regionals competition and the "Funny Girl" audition.)

And now, in celebration of Glee's two-part 100th episode, Rachel (Lea Michele), Kurt (Chris Colfer), and the rest of the characters that we have come to know and love, will once again take the auditorium stage to sing their anthem of strength and optimism.

Since we love Glee just as much as you do, we're bringing you an exclusive sneak peek of Tuesday night's grand finale that will serve as the series' catalyst into a solely New York-centric storyline.

MORE: Catch up on last week's episode of Glee!

With the future of the glee club still looking bleak, Will (Matthew Morrison), the WMHS alumni and the current New Directions will continue to band together in Tuesday's 101st episode in an attempt to save McKinley's music.

In addition to "Don't Stop Believin'" Tuesday's episode, "New Directions," will also feature "I Am Changing" from Dreamgirls, Glee's original hit, "Loser Like Me," and many more.  Glee: The Music, Celebrating 100 Episodes will be an album accompanying the music from ep 100 and this episode, as a celebration of the show's special milestone. The album will be released March 25, 2014.

Glee airs Tuesdays at 8 p.m. on Fox.