Beyonce, Jay Z, GLAAD Media Awards

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Beyoncé's 2019 GLAAD Media Awards speech was irreplaceable. 

It wasn't long before the global superstar had guests cheering after taking the stage alongside her famous husband, Jay-Z, to accept the prestigious Vanguard Award on Thursday night in Los Angeles. 

During the star-studded ceremony, the 23-time Grammy-winning songstress told the crowd she was "overwhelmed" as she delivered an impassioned speech following a medley performance by Shangela as well as an introduction from Morris Singletary, Janet Mock, Ty Hunter and Lena Waithe

"We were not expecting any of those incredible presenters and just super honored and overwhelmed. I have already cried. I put a run in my stocking," the mother of three told the crowd as she began.

"I would say that one of the most beautiful memories from our tour was looking out every night and seeing the hardest gangster trapping out right next to the most fabulous queen, full out, respecting and celebrating each other. That was dope! That's the beauty of great partnership, connecting people who at first glance seem worlds apart," she continued at the event, sponsored by Ketel One Family-Made Vodka.

"Whether it's our fans, or our family, the LGBTQI community has always supported us and lifted us up. We're here to promote love for every human being, and change starts with supporting the people closest to you. So, lets tell them they are loved. Let's remind them they are beautiful. Let's speak out and protect them. And parents, let's love our kids in their truest form."

As the triple threat continued, she made a plea. "We would like to request that we continue to shift the stigma in this community, especially the stigmas in black families, towards accepting queer black and brown family members," she said. 

The star then grew emotional as she honored her late uncle, who passed away from HIV. "‪Lastly, I want to dedicate this award to my Uncle Johnny, the most fabulous gay man that I have ever known...who helped raise me and my sister. He lived his truth. He was brave and unapologetic during a time when this country wasn't as accepting, and witnessing his battle with HIV was one of the most painful experiences I've ever lived," she said, her voice beginning to tremble. Jay-Z rubbed her back to comfort her.

"‪I am hopeful that his struggle served to open pathways for other young people to live more freely. LGBTQI rights are human rights. To choose who you love is your human right. How you identify and see yourself is your human right. Who you make love to and take that ass to Red Lobster is your human right," she continued, the audience cheering and laughing. 

‪She then turned her attention to the rapper, telling him, "I just want to say to you, Jay, that I am so proud of you for making incredible strides towards changing stigmas in the hip-hop community. It's a privilege to watch you take those steps and to stand right next to you. I love you and we're super grateful and honored."

The "Story of O.J." star used his time at the microphone to recognize his mother, Gloria Carter, who was honored at last year's ceremony.  

"Life, this journey, is filled with highs, lows, and a lot of learning. This is a momentous night. I also want to, because I didn't get to do it last year, honor my mother, who received the award last year, and follow in her footsteps of spreading love and acceptance and her beautiful speech at the end of the song "Smile," for her allowing me to tell her story," he said. "So, I want everyone to acknowledge that, and her strong message of love who you love and souls that connect."

See more of the night's famous attendees in E!'s gallery below. 

Reporting by Spencer Lubitz

Michelle Kwan, Hayley Kiyoko, Jonathan Van Ness, Candids, 2019 GLAAD Media Awards

Rachel Murray/Getty Images for Ketel One Family-Made Vodka

Gwyneth Paltrow, 2019 GLAAD Media Awards

Michael Kovac/Getty Images for Ketel One Family-Made Vodka

Lea Michele, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Adam Rippon, 2019 GLAAD Media Awards

Matt Winkelmeyer/Getty Images for GLAAD

Jonathan Van Ness, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Allison Janney, Sean Hayes, Jamie Lee Curtis, Adam Lambert, 2019 GLAAD Media Awards, Candids

Kevin Mazur/Getty Images for GLAAD

Meghan Trainor, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Jazz Jennings, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Jameela Jamil, Tan France, 2019 GLAAD Media Awards, Candids

Michael Kovac/Getty Images for Ketel One Family-Made Vodka

Antoni Porowski, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Michelle Kwan, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Jameela Jamil, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Pamela Stewart, Lena Waithe, 2019 GLAAD Media Awards, Candids

Matt Winkelmeyer/Getty Images for GLAAD

Gina Gershon, Jennifer Tilly, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Erika Jayne, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Dita Von Teese, 2019 GLAAD Media Awards

Matt Winkelmeyer/Getty Images for GLAAD

Lea Michele, Meghan Trainor, 2019 GLAAD Media Awards, Candids

Kevin Mazur/Getty Images for GLAAD

Ross Mathews, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Allison Janney, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

 Lauren Ash, Nico Santos, Nichole Bloom, 2019 GLAAD Media Awards, Candids

Rachel Murray/Getty Images for Ketel One Family-Made Vodka

Olivia Munn, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Shangela, 2019 GLAAD Media Awards

Rich Fury/Getty Images for GLAAD

Adam Lambert, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Shangela, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Melissa Fumero, Stephanie Beatriz, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Sean Hayes, Scott Icenogle, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Josh Duhamel, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Todrick Hall, Adam Rippon, Jussi-Pekka Kajaala, 2019 GLAAD Media Awards, Candids

Michael Kovac/Getty Images for Ketel One Family-Made Vodka

Lea DeLaria, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Rachel Bloom, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Dan Reynolds, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Tan France, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Gigi Gorgeous, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

EJ Johnson, 2019 GLAAD Media Awards

Rich Fury/Getty Images for GLAAD

Meghan Trainor, Queer Eye Cast, GLAAD Media Awards, Candids

Kevin Mazur/Getty Images for GLAAD

Janet Mock, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Bobby Berk, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Jenifer Lewis, 2019 GLAAD Media Awards

Broadimage/REX/Shutterstock

Gina Gershon, Jennifer Tilly, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Hayley Kiyoko, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Logan Browning, 2019 GLAAD Media Awards

Kevin Mazur/Getty Images for GLAAD

Nick Robinson, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Barbara Corcoran, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Lauren Ash, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

Trace Lysette, 2019 GLAAD Media Awards

Frazer Harrison/Getty Images

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