The 28-year-old royal will be joined by True Blood hunk Alexander Skarsgård, who has joined the American team and The Wire's Dominic West, who is going with the Commonwealth team.
Each team is raising funds to military charities from their respective nations—Soldier On (Australia and Canada), Soldiers to Summit (United States of America) and Walking with the Wounded (United Kingdom).
According to Kensington Palace, the teams will depart London for Cape Town, South Africa on Sunday, Nov. 17. They will then head to Novolazarevskaya Station in Antarctica on Nov. 19, where all participating groups will spend a few days adjusting to the Antarctic temperatures.
Once they've gotten their bearings, the teams will fly 87 degrees south to spend even further time acclimating to the temperatures.
The official trek begins on Nov. 30 and Kensington Palace officials hope that the groups reach their South Pole destination by Dec. 16.
All participating team members will race 208 miles across the Antarctic Plateau and are expecting to travel approximately 10 miles a day. Keep in mind, they'll also be carrying extremely heavy backpacks with them throughout the entire excursion.
Temperatures are said to be as low as 49 degrees below zero Fahrenheit, with winds up to 50 mph, during some points of the charity mission.
But worry not, each team will be guided by an experienced hiker and mentor as well as an emergency medical team.
Prince Harry introduced members of the U.K. team to Queen Elizabeth II Wednesday just before he departed for the journey.
Hikers showed Harry's grandmother one of the warmest coats and explained other key items of equipment that they'll be taking.
The rugged royal isn't exactly new to extreme conditions like Antarctica either. He took part in a Walking with the Wounded trek to the North Pole in 2011.