They don't call this the Legends of the Summer tour for nothing.
The "Suit & Tie" duo saved the last song of their concert for the tribute, with JAY Z and Timberlake performing a rendition of "Forever Young" in honor of the late teen.
"This is for Trayvon, so light up your cell phones New York!," JAY Z yelled, with Timberlake adding, "One more time for Trayvon New York!"
In addition to their tribute, the pair brought along a couple of surprise performers, with Alicia Keys popping up during JAY Z's performance of "Empire State of Mind," and Timbaland hitting the stage for Timberlake's "SexyBack."
And after the show was the after party.
JAY Z, Beyoncé, Neyo, Meek Mill, and Fabolous all arrived at about 1:30 a.m. to the rap star's 40/40 club in New York City, with the crew sipping JAY Z signature tour cocktails courtesy of D'Ussé Cognac at their VIP table to celebrate JAY Z's first stop on his U.S. tour, a source told E! News exclusively.
Beyoncé was dressed very casual at the club, rocking curly hair, and sporting a button down long sleeve shirt with shorts and Christian Louboutin stilettos.
The husband-and-wife duo was spotted grabbing some food before the night was done, and Beyoncé got her groove on throughout the night, dancing with friends.