During his performance of the hit song on the NBC sketch show, Timberlake changed the lyrics from "Aww, s--t so sick, got a hit and picked up a habit" to "my hit's so sick, got rappers acting dramatic."
While it was a subtle dig, Timberlake seemed to be addressing the comments West made at a London concert in February.
"I got love for Hov, but I ain't f--king with that 'Suit & Tie,'" West told the crowd. We're sure this burgeoning feud is a bit awkward for Jay-Z: The rapper released a joint album with West, "Watch the Throne," and is set to embark on a stadium tour together this summer.
Either way, Timberlake is earning rave reviews for his fifth outing as Saturday Night Live's host, so we're sure West's dig didn't get to him too much.
(E! and Saturday Night Live are a part of the NBCUniversal family.)
What did you think of Timberlake's diss? Sound off in the comments!